About Breath Testing

How to Test for SIBO Easily At Home

Find out how to easily test for SIBO at home.

My SIBO Checklist


“The SIBO breath test was very easy to use. I loved the fact I could do it from home and my practitioner had the results in a very short amount of time”

What Is Included with My Test?

  • Breath Collection Device
  • 10 Test Tubes
  • Sugar Substrate
  • Detailed Instructions
  • Detailed Report

Completing My SIBO Breath Test



Order a SIBO Breath Test here

Easy and secure online ordering. Your SIBO Test is shipped within 1 business day. You'll be able to track your package worldwide via Australia Post.


Ensure you leave enough time for the SIBO Prep Diet

1-2 days for samples from each kit.


Complete test and return breath samples to SIBOTest.

Once we have received your samples, turnaround time is 1-2 business days for your test results.


Receive your test results from your practitioner or directly via SIBOTest.com

We provide results to your practitioner (if you have nominated one) or directly to your account.

Our Tests

SIBO BASIC: Lactulose Only

AUD $175 + Shipping + GST for relevant orders.

The SIBO Basic test uses lactulose as the test substrate. Lactulose breath testing for the diagnosis of SIBO is widely used by medical practitioners and a good place to start when assessing for SIBO. The lactulose breath test will provide a conclusive result in most cases.
Note to Practitioners: Order this test when you highly suspect SIBO in your patients. We are the only lab to offer Lactulose breath testing as the sole test to help you diagnose SIBO. Other labs use Glucose as the only diagnostic test sugar and Lactulose as the control sugar.

See below as to why we think glucose is not the best substrate to diagnose SIBO. Using glucose only as the test substrate can miss many SIBO positive cases.

Lactulose breath testing for the diagnosis of SIBO is widely used by medical practitioners in the USA. Dr Mark Pimentel, Director of the Motility Program in the Gastroenterology Department of Cedars-Sinai Hospital in Los Angeles, uses this test exclusively to diagnose SIBO. He currently has done over 19,000 Lactulose breath tests and believes this test has a high level of accuracy when interpreted correctly. He is the author of “A New IBS Solution” and considered the foremost expert on SIBO.

A double-blind randomized study* revealed that 84% of IBS patients exhibited bacterial overgrowth based on lactulose breath testing.

* Am J Gastroenterol. 2003 Feb;98 (2):412-9. Normalization of lactulose breath testing correlates with symptom improvement in irritable bowel syndrome. a double-blind, randomized, placebo-controlled study. Pimentel M, Chow EJ, Lin HC.

SIBO ADVANCED: Lactulose + Glucose

AUD $299 + Shipping + GST for relevant orders.

The SIBO Advanced breath test comprises of two test kits,using substrates lactulose and glucose. Using both lactulose and glucose provides an added assurance for the correct diagnosis of SIBO. Some practitioners prefer ordering this combination test as an extra confirmatory of diagnosis. Order this test when your patient has a more complex symptom picture with possible multiple diagnoses. Because not all bacteria ferment lactulose, the addition of glucose provides further assurance of the diagnosis of SIBO.


SIBO COMPREHENSIVE: Lactulose + Glucose + Fructose

AUD $409 + Shipping + GST for relevant orders.

The SIBO Comprehensive test comprises of three test kits and uses substrates lactulose, glucose, and fructose. As the name implies, this is the most comprehensive test available for the assessment and diagnosis of SIBO. This triple test approach has the highest likelihood of finding fermentation and gas production if it is present. Additionally, if the test is positive for SIBO, it can show if fructose needs to be avoided during the treatment diet. Order this test if in addition to SIBO, fructose malabsorption is suspected.


AUD $189 + Shipping + GST for relevant orders.

The fructose malabsorption test is for those not suspected of having SIBO but are experiencing symptoms with consumption of fruit, honey, and other fructose containing foods. SIBOTEST offers a more thorough assessment of fructose malabsorption by using a ten sample test, with samples collected every 20 minutes over a 3 hour period.

What Is The SIBO Breath Test?

The breath test is a simple, non-invasive test. After a 24 hours preparatory diet, the lactulose (or glucose or fructose) test substrate is swallowed.

Lactulose is not absorbed and therefore acts as a food source for bacteria, if present, in the small intestine.

The bacteria ferment the testing substance and produce hydrogen and/or methane. These are diffused into the bloodstream and exhaled via the breath.

Breath samples are collected every 20 minutes for three hours. A third gas, hydrogen sulphide, can also be present but cannot be assessed via breath testing at this time.

All our tests use machines from QuinTron, the original manufacturer of BreathTracker, to measure both hydrogen and methane in a single sample of breath (alveolar air)

Testing for SIBO using the QuinTron BreathTracker is considered gold standard.

Extensive quality control, including carbon dioxide measurements to assure an acceptable alveolar air sample, comply with the highest standards of analytical excellence.

Watch this short video about why it's important to test, and not just treat, for SIBO.

Important Preparation

24 hours (48 hours if you are constipated) before you start the test, you MUST restrict certain foods. You must also avoid certain medications and supplements for a minimum of 1 week.

Please download and read the preparation instruction booklet



  • How long does it take to get my test results?
  • Results will be made available to your nominated practitioner in 1-2 business days after we receive your test samples.
  • Is the lactulose breath test alone a reliable indicator of SIBO?
  • This is a hotly debated topic in gastroenterology research at the moment and research for and against using the lactulose breath test alone is being published in increasing numbers. SIBOtest closely follows Dr Mark Pimentel's recommendations for lactulose breath testing in the diagnosis of SIBO. Dr Pimentel, Head of Motility at Cedars Sinai Hospital in the USA, has conducted over 19,000 lactulose breath tests for the diagnosis and treatment of SIBO. His argument that lactulose breath testing is a viable test of SIBO is as follows: After a dose of 10g of Lactulose is given, 5% of Lactulose arrives in the cecum after 1 hour. Therefore 95% of it is still in the small instestine. Once it arrives in the cecum it takes colonic bacteria another hour to ferment the arriving lactulose. Therefore it takes a minimum of 2 hours for the colonic fermentation to affect the breath test.Test results are based on the first 2 hours depending on the patient's transit time. In the USA, many labs offer Lactulose breath testing as the test to diagnose SIBO-- even the SIBO Centre in Portland, Oregon has confidence in this method. Unfortunately, there is no perfect test. False positives are rare and usually caused by improper preparation or collection. Almost all false negatives are avoided by measuring methane in addition to hydrogen. SIBOtest also offers glucose as the test sugar for practitioners wanting additional confirmation in the diagnosis of SIBO.
  • Can I do a breath test after a colonoscopy has been performed?
  • Please allow at least a month after a colonoscopy.
  • Can the breath test be done later in the day: eg late morning?
  • Yes. Remember though that water is only to be consumed prior to testing.
  • Re the Diet Instruction sheet for lactulose. Is this instruction sheet the same for glucose?
  • Yes. They are the same diet instructions for both tests, however the glucose test requires different testing substance mixing instructions
  • I take movicol every evening a few hours after dinner. Can I still take this (night before).
  • No. Unfortunately, we do not want anything that may interfere with the actual testing result. Any laxative use the day before you start testing can influence the test results.
  • I am doing more than one test: lactulose, glucose and fructose. Can I do the tests separately (eg a week apart) or should they be done on consecutive days?
  • Yes, you can do the testing in this way, although it’s a lot easier to do on consecutive days due to the diet preparation. If you do complete the tests separately, please return tests within 3 days of completion.
  • What order should I do the tests if I am doing multiple tests?
  • Lactulose, glucose and then fructose. Resume prep diet after each test, fast for 12 hours overnight and complete the next test the next morning. Do not complete them on the same day.


  • On the prep diet, can I season the broths with vegetables and strain them?
  • No. No vegetables are allowed. No stock powder. Only real meat can be used to make soup/broth.
  • On the prep diet, can I use olive oil or any oil in cooking?
  • A small amount of oil for cooking is allowed.
  • Some of the tubes look funny. Is this normal?
  • During the manufacturing process the tubes are punctured in order to remove the air inside to create a vacuum. Sometimes you can see this puncture mark, and sometimes you cannot. In addition, the rubber stopper on some of the tubes may appear concave, while appearing convex on others. This too is a normal part of the manufacturing process.
  • Am I supposed to remove the grey rubber cover from the needle on the collection device?
  • This rubber sheath provides an additional seal against the rubber stopper of the test tube. Please do not remove it.
  • There are holes in my blue collection bag. Should I seal them with tape?
  • These holes are designed to vent the initial portion of your breath. Covering these holes may actually dilute your samples.
  • Should I be able to see my breath inside of the tube?
  • You will not be able to see the air go into the tube, nor will you see any condensation.
  • Does my test kit expire?
  • Yes. Please check the expiration date located on the side of the test kit box


The SIBO breath test was so simple to use and now I have the information I needed to better my health and vitality.

After so many years of misdiagnosis SIBOTest has made a massive difference in my health and my outlook. I now run a support group.

Thank you SIBOTest for your very helpful and friendly customer support and all the great resources available.

Do I have SIBO?

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